How do you make chloramphenicol stock solution?

Directions:

1. Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol.
2. Filter through a 0.22 µm filter to sterilize.
3. Aliquot and store at -20°C.
4. Use at 1:1000 dilution in LB or LB-Agar.

Add 10 ml of 95% EtOH (70% may also be used).

We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml of 70% ethanol and mix the content by rotating the tube. Once the content is mixed, make up the volume to 10 ml.

Stock may be kept At -20°C for 1 year.

Chloramphenicol – The frozen stock solutions of chloramphenicol are at 25mg/ml in 100% ethanol and are marked with purple. The final concentration for LB liquid culture is 25mg/ml. To obtain this in 100ml of LB, add 100ul stock solution.

Chloramphenicol should be administered intravenously as a 10% (100 mg/ml) solution or lower, that can be prepared by Diluting the lyophilized powder in the vial with 9.2 ml of diluent (see table below). The resulting solution can be administered as an intravenous bolus, intravenous infusion or intramuscular injection.

Directions:

1. Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol.
2. Filter through a 0.22 µm filter to sterilize.
3. Aliquot and store at -20°C.
4. Use at 1:1000 dilution in LB or LB-Agar.

Preparation of 80ml stock solution

1. Tetracycline is kept in the 4C fridge in 68-564D. …
2. Weigh 400mg of tetracyline HCL into a small weigh boat.
3. Dilute 95% Ethanol to 70% using milliQ water. …
4. Add 80ml of 70% Ethanol to a 250ml bottle.
5. Add the tetracycline HCL to the ethanol.

Antibiotic Concentrations

Chloramphenicol is soluble in ethanol (50mg/ml), butanol, acetone and ethyl acetate, Slightly soluble in water (2.5mg/ ml) and insoluble in benzene, petroleum ether and vegetable oil.

To prepare 50 mg/mL BCIP disodium salt, Dissolve 0.5 g in 10 mL H₂O.

It is suggested the stock solution should be stored Refrigerated And used within 30 days.

Eye drops can be kept in a cupboard for up to 7 days but it is better to keep them in a fridge. Make sure that the medicine doesn’t freeze. The drops should be taken out of the fridge 2 hours Before using them, so that they do not sting.

You can add chloramphenicol with an average of 0.05 gm. L^–1 To PDA medium Before autoclaving because the chlramphenicol is not be affected by heating and then put the medium in autoclave at 121 ⁰C and 15 pound. inch ^–1 For 20-30 minutes. After cooling to about 45 ⁰C, medium was poured in Petri dishes.

You can add chloramphenicol with an average of 0.05 gm. L^–1 To PDA medium Before autoclaving because the chlramphenicol is not be affected by heating and then put the medium in autoclave at 121 ⁰C and 15 pound. inch ^–1 For 20-30 minutes. After cooling to about 45 ⁰C, medium was poured in Petri dishes.

Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars. The widely used rich medium called Luria-Bertani broth is popular with bacteriologists because it Permits fast growth and good growth yields for many species.

The major toxicity of chloramphenicol is hematological. In all vertebrates studied, It produces direct, dose-dependent bone marrow depression resulting in reductions in red blood cells, white blood cells, and platelets.

Chloramphenicol is a widely used topical ointment applied routinely to suture lines and skin grafts, particularly those on the face and around the eyes.

Toxicity. Chloramphenicol can be fatal in an overdose; this usually occurs with intravenous administration of the drug and is more likely to affect infants. Symptoms of poisoning include Nausea and vomiting, abdominal distension, metabolic acidosis, hypotension, hypothermia, cardiovascular collapse, and coma.

Preparation of 500 – 1000X stock at 50 mg/ml: 1) Dissolve 5 grams of Carbenicillin disodium salt in 100 ml distilled water. 2) Vortex to make sure completely soluble. 3) Sterile by filtering through a 0.2 µm filter. 4) Aliquot and store at -20 ⁰C up to six months.

Ampicillin : Add 1ml ampicillin (at 100mg/ml) per liter of agar To obtain a final concentration of 100ug/ml.
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All Answers (3)

1. Mix 1 g Amp powder into 10 mL of d H2O.
2. Vortex until powder is thoroughly dissolved.
3. Sterilize using 0.22-μm sterile filter.
4. Aliquot 1 mL of Amp solution per 1.5 mL tube and store at -20 °C.

Dissolve 1 g of sodium ampicillin in sufficient H₂O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H₂O. Then pass the ampicillin solution through the washed filter.

Amoxicillin is poorly soluble in water, even less so in ethanol. Try dissolving in 0.1M NaOH/PBS (1:1 v/v). That should give a weak alkaline solvent.

For oral dosage forms (capsules and suspension): Adults and teenagers—Dose is based on body weight. The usual dose is 12.5 milligrams (mg) per kilogram (kg) (5.7 mg per pound) of body weight every six hours.

Preparation of 80ml stock solution

1. Tetracycline is kept in the 4C fridge in 68-564D. …
2. Weigh 400mg of tetracyline HCL into a small weigh boat.
3. Dilute 95% Ethanol to 70% using milliQ water. …
4. Add 80ml of 70% Ethanol to a 250ml bottle.
5. Add the tetracycline HCL to the ethanol.